Sensitive Deep Sequencing-based HIV-1 Genotyping Assay to Simultaneously
نویسندگان
چکیده
Running Title: Deep Sequencing-based HIV-1 Genotyping and Coreceptor Tropism Assay 5 6 7 Richard M. GIBSON , Ashley M. MEYER , Dane WINNER , John ARCHER , Felix FEYERTAG , 8 Ezequiel RUIZ-MATEOS , Manuel LEAL , David L. ROBERTSON , Christine L. SCHMOTZER , and 9 Miguel E. QUIÑONES-MATEU 1,4,* 10 11 1 University Hospital Translational Laboratory, University Hospitals Case Medical Center, Cleveland, Ohio, USA; 12 2 Computational and Evolutionary Biology, Faculty of Life Sciences, University of Manchester, Manchester, United 13 Kingdom; 3 Laboratory of Immunovirology, Biomedicine Institute of Seville, Service of Infectious Diseases, Virgen 14 del Rocio University Hospital, Seville, Spain; and 4 Department of Pathology, Case Western Reserve University, 15
منابع مشابه
Contribution of Human Immunodeficiency Virus Type 1 Minority Variants to Reduced Drug Susceptibility in Patients on an Integrase Strand Transfer Inhibitor-Based Therapy
The role of HIV-1 minority variants on transmission, pathogenesis, and virologic failure to antiretroviral regimens has been explored; however, most studies of low-level HIV-1 drug-resistant variants have focused in single target regions. Here we used a novel HIV-1 genotypic assay based on deep sequencing, DEEPGEN (Gibson et al 2014 Antimicrob Agents Chemother 58∶2167) to simultaneously analyze...
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Recent advances in genotyping technologies have allowed for detection of HIV-1 drug resistance mutations present at low levels. The presence and percentage of Y181C and K103N drug-resistant variants in the blood of 105 subtype C HIV-infected infants who failed single-dose nevirapine prophylaxis for HIV transmission were compared using two highly sensitive genotyping methods, allele-specific PCR...
متن کاملRunning Title: Deep Sequencing-based HIV-1 Genotyping and Coreceptor Tropism Assay
Running Title: Deep Sequencing-based HIV-1 Genotyping and Coreceptor Tropism Assay 5 6 7 Richard M. GIBSON , Ashley M. MEYER , Dane WINNER , John ARCHER , Felix FEYERTAG , 8 Ezequiel RUIZ-MATEOS , Manuel LEAL , David L. ROBERTSON , Christine L. SCHMOTZER , and 9 Miguel E. QUIÑONES-MATEU 1,4,* 10 11 1 University Hospital Translational Laboratory, University Hospitals Case Medical Center, Clevela...
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